Persons with CF are prone to chronic infections and hyperinflammatory responses, which are associated with progressive tissue damage. It is now recognized that chronic infection and inflammation results from intrinsically dysfunctional immune cells. Therefore, the overarching goals of the C3 Immune Core are to provide a unified source of fresh immune cells to investigators, deliver reproducible data, and enable training of students and fellows in CF immune cell functions. C3IC services support projects and C3 members who seek to understand complex CF host-pathogen interactions and inflammation and thereby develop novel therapeutic strategies that prevent the establishment of chronic infections and tissue destruction.
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C3IC, in collaboration with the C3 Translational and Data Core, provides sample collection and analysis services for clinically derived specimens. Click the 'REQUEST SPECIMENS' button to submit a request, or learn more about the specimen request process by visiting the Translational and Data Core webpage.
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IC Facilitates Research Through:
- Isolation and analysis of fresh blood immune cells (neutrophils, lymphocytes, peripheral blood monocyte-derived macrophages), fresh bronchoalveolar lavage-derived immune cells, and fresh sputum-derived immune cells;
- Critical assays of immune function including comprehensive cell counts, cytokine/chemokine production, and Enzyme-Linked ImmunoSpot (ELISpot) assays from immune cells at baseline and in response to infections or treatments;
- Functional analysis including halide efflux and patch-clamp electrophysiology for CFTR and other ion channels within immune cells;
- Provides expertise and training in use of the Agilent Seahorse for analysis of oxygen rate and proton efflux rate of live immune cells; and
- Access and assistance with interpretation of state of the art ‘omics assays (single-cell RNA-Seq, mi-RNA-Seq, Methyl-Seq, CyTOF, metabolomics, proteomics) in immune cells.
Acknowledging C3IC
Publish Results
Publications resulting from studies supported by C3IC should include the following acknowledgement:
Publications resulting from studies supported by C3IC should include the following acknowledgement:
This work was supported in part by the Cure CF Columbus Immune Core (C3IC). C3 is supported by a Cystic Fibrosis Foundation Research Development Program Grant (MCCOY17R2), the NCH Division of Pediatric Pulmonary Medicine (MCCOY19RO), and the OSU Center for Clinical and Translational Science (UL1TR002733).